Dear colleagues,
I am trying to develop metastasis in nude mice using COLO-205 cancer cells (RFP positive). I have injected 1 million cells in PBS to each mouse via tail vein. After 6-7 weeks, I observed many cutaneous tumors like mass around the body (5 out of 5 mice). After dissection, these tumors appeared nearly spherical in shape and with visible blood vessels. I made single cells from these tumors and found they are RFP positive. Is these tumors are derived form COLO-205..? Is this is common phenomenon….? Have anybody observed similar results…? Looking for suggestion
There is a possibility that dissemination of tumor cells occur, but given the "seed and soil theory" in the development of the colorectal tumor, it is unlikely that those malignant cells show the metastasis exclusive to the skin.
You used the method of experimental metastasis. It works as a method in your system.
