I need simple protocols for collagen extraction from
human placenta, rat tail, egg shell and rabbit skin
Dear Sir. Concerning your issue about the collagen extraction from human placenta, rat tail, egg shell and rabbit skin. There is a growing interest in the extraction process of collagen and its derivatives due to the growing tendency to use this protein to replace synthetic agents in various industrial processes, which results in a greater appreciation of the by-products from animal slaughter. Collagen’s characteristics depend on the raw material and the extraction conditions, which subsequently determine its application. The most commonly used extraction methods are based on the solubility of collagen in neutral saline solutions, acid solutions, and acid solutions with added enzymes. Recently, the use of ultrasound, combined with these traditional processes, has proven effective in increasing the extraction yield..I think the following below links may help you in your analysis:
http://www.ifrj.upm.edu.my/23%20(03)%202016/(1).pdf
http://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.16.pdf
http://www.hej.kk.usm.my/pdf/HEJVol.4No.2/Article04.pdf
Thanks
Dear Dr. Hiba
There are number of protocols for collagen extraction from various animal tissues. However, the one you choose will depend primarily on your aim and intended application.
You will find attached to this answer some pdf files which include some helpfull information.
With best wishes
Pepsin digestion yields atelocollagen-1 and 3 at rather high yield (300-500mg dry weight per 100g wet weight tissue). Acid yields tropocollagen at rather low yield, but eg a higher transparancy...
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