Hi,
Does anyone have first hand experience of using pETDuet-1 vector and pRSFDuet-1 vector. Both of them have two promoter followed by two MCS. Does both of them offer similar level of expression or is there any bias?
In designing a clinical trial, it is necessary to consider a false pressure point for sham groups. Given that the main pressure point is around the lumbocostal region, what specific point or...
26 May 2023 8,250 1 View
Hi, I am trying to express a gene which belong to classical TA (Toxin-Antitoxin) module. Specifically, I am trying to express the toxin gene using pBAD vector in BL21 cells. Despite trying out...
08 March 2022 8,311 2 View
Hi, I am planning to tag a gene endogenously (in vivo) in E. coli for immunoprecipitation. Can anyone suggest an elegant protocol for this? Thanks!
29 June 2021 7,884 1 View
Hi, I am traying to optimize the expression level of my protein cloned in pBAD vector. Currently, I am inducing it at 0.002 w/v but see a significant overexpression of my protein. The current...
26 May 2021 6,335 2 View
Hi, Does anyone know of any plasmid where I can clone two genes under two promoters? Basically, I want to coexpress these protein together but with Arabinose inducible system. pETDuet,...
07 May 2020 8,328 0 View
Hi, Is there any way to prove that a protein is membrane anchored/peripheral membrane protein? Any simple trick or any technique will be helpful. Thanks
24 November 2019 1,152 5 View
Hi, Can someone please suggest which detergents to use in regular lysis buffer/resuspension buffer for E. coli cells? Please also suggest the optimum concentration to be used. Thanks.
24 November 2019 1,485 5 View
Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...
21 March 2019 3,201 4 View
Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...
17 December 2018 8,644 2 View
Hi, Can anyone please highlight the difference between normal agarose and agarose low EEO? Any specification as to which to use for agarose gel electrophoresis? Is it that extraction of pcr band...
30 November 2018 6,802 3 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I have designed magnetic material with different biasing conditions in HFSS. Now I want to give an RF AC signal and do a transient simulation in HFSS. Is it possible to do in HFSS? Please help me...
24 July 2024 9,319 5 View
Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...
20 July 2024 1,943 1 View
I want to know that n-doped side of solar cell is connected with positive or negative electrode.
20 July 2024 1,348 2 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
When testing devices based on ITO/ZnO/CsPbBr3/TFB/MoO3/Ag structures, I found that the current density of the device always increases sharply to 1000mA/cm2 at about 3V bias, and the device does...
16 July 2024 4,291 0 View