FLAG tag Immunoprecipitation?

30 June 2021 1 8K Report

Hi,

I am planning to tag a gene endogenously (in vivo) in E. coli for immunoprecipitation. Can anyone suggest an elegant protocol for this? Thanks!

Gloria Volohonsky

Keep in mind that FLAG tag is very good for analysis, but not so useful if you want to purify your protein.

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