Has anyone dealt with technical issues with both primary rat neuron/glia co cultures and human iPSC neuron astrocyte co cultures (both on PDL), where the cells seem to ball up near the cell body and cause long filament string like processes to one another? We think it could be due to the coating or a cell signaling process? See image comparison of healthy vs. "spindled" like cells. Apologies for the bad quality image due to uploading on here.

Any technical advice welcome. Thank you!

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