Clear Native-PAGE for in-gel activity assay

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Has anyone done proteasome assay in 96 well plate format.. I did for 1st time n it didnt workout. Many wells giving zero reading.. can anyone help

tied doing Proteasome assay using AMC conjugated substrates... However many sample wells gave zero reading.. infact the samples i ran in duplicate they are showing strange readings.. one well...

10 November 2011 9,142 0 View

Band at same position using two different antibodies

I ran 12% SDS PAGE using tris glycine buffer. after transfer i cut two lanes for ubiquitin detection and 4 lanes for beta actin. after development i got a strong intense single band for b actin....

01 February 2011 9,957 7 View

In gel assay for proteasome activity

Hi i have been trying to standardise this method for long but not getting good results. I am using 4-8% gradient gels with running buffer composed of tricine and bis-tris. i dont get separation of...

31 December 2010 1,065 7 View

Proteasome assay

Hi I wish to do the proteasome assay using the flouregenic substrates Suc-LLVY-AMC, Z-LLE-AMC, Boc-Leu-Arg-Arg-AMC. All the protocols i have read are using well plates. But my spectroflourometer...

06 July 2010 1,198 4 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View