Poonam Thakur

5 Questions 15 Answers 0 Followers

Questions related from Poonam Thakur

Has anyone done proteasome assay in 96 well plate format.. I did for 1st time n it didnt workout. Many wells giving zero reading.. can anyone help

tied doing Proteasome assay using AMC conjugated substrates... However many sample wells gave zero reading.. infact the samples i ran in duplicate they are showing strange readings.. one well...

11 November 2011 9,176 0 View

Band at same position using two different antibodies

I ran 12% SDS PAGE using tris glycine buffer. after transfer i cut two lanes for ubiquitin detection and 4 lanes for beta actin. after development i got a strong intense single band for b actin....

02 February 2011 9,992 7 View

In gel assay for proteasome activity

Hi i have been trying to standardise this method for long but not getting good results. I am using 4-8% gradient gels with running buffer composed of tricine and bis-tris. i dont get separation of...

01 January 2011 1,095 7 View

Proteasome assay

Hi I wish to do the proteasome assay using the flouregenic substrates Suc-LLVY-AMC, Z-LLE-AMC, Boc-Leu-Arg-Arg-AMC. All the protocols i have read are using well plates. But my spectroflourometer...

07 July 2010 1,225 4 View

Clear Native-PAGE for in-gel activity assay

Hi I need a protocol for clear native NAGE. i need it to perform in-gel activity asay for electron transport chain complex lll. Also if anyone could help me with providing a protocol for this...

07 July 2010 9,239 2 View