Has anyone done proteasome assay in 96 well plate format.. I did for 1st time n it didnt workout. Many wells giving zero reading.. can anyone help

11 November 2011 0 9K Report

tied doing Proteasome assay using AMC conjugated substrates... However many sample wells gave zero reading.. infact the samples i ran in duplicate they are showing strange readings.. one well gives zero while other gives some reading... Pipeting has been done very carefully.. can anyone please suggest the reason.. Has anyone done the assay or similar assay like calpain asssay.??? please explain the exact methodology followed

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