Dear Experts,
I am a beginner in Lateral Flow Assay. I am doing the conjugation. It appears that there are many kinds of gold nanoparticles to choose: bare particles, Carboxyl coated or NHS coated particles. I do not know which kind should I start with. As far as I know, the bare gold nanoparticle is a traditional one and the protocol is not difficult to do but the attachment maybe dissociate.
Thank you for your kind help!
Shao-Hsuan Kuo Thank you. Is a covalent nanoparticle is better than the passive adsorption one regarding the attachment to antibodies?
Passive adsorption is enough for functionalization and stabilization. Covalent attachment is a pain in the ass.
The NHS group quickly hydrolyzes in water, the same is true for carbodiimide chemistry (the reactive group quickly hydrolyzes in water). Another challenge is to keep the nanoparticles stable when trying covalent modification. Passive adsorption does the job, you still need to prevent non-specific adsorption when running the lateral assay.
Omar Gonzalez-Ortega Gracias Professor. It's very kind of you to answer all the questions. I really appreciate your help. I have some more questions but I should explore by my self first.
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