Hi guys,
I am new to transfected CHO cell recordings and I've noticed that my membrane capacitance has a 50% rundown within 3 min of recording before stabilizing. Cell seem healthy and seal remains good. Do you guys see the same thing?
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
27 February 2021 2,753 4 View
I want to get a average structure from a pdb file containing multiple models. I used the command below: gmx cluster -f input.pdb -s topol.tpr -cutoff 20 -method gromos -av GROMACS...
25 February 2021 6,314 1 View
The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...
18 February 2021 9,538 1 View
Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...
15 February 2021 8,982 2 View
Hi I've been looking for a stat package for my sensory evaluation data (progressive profiled which is a multi-timepoint version of descriptive analysis). Is there any available one to...
08 February 2021 9,170 3 View
Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...
27 January 2021 2,525 4 View
Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...
27 January 2021 7,924 1 View
Dear researchers, I am modeling the process of the water droplet impacting on the wall with a constant contact angle. The specific calculation parameters are as follows: The droplet diameter is...
24 January 2021 1,020 5 View
Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...
07 January 2021 5,610 4 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
28 February 2021 2,534 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View