Dear Forbes Avila ,

I think this protocol can be helpful for your work,

Coated of isoniazid with calcium alginate

Isoniazid weighed 0.1 g was dissolved in 100 mL distilled water containing methanol (methanol/water 1:4 v/v). The sodium alginate 2% (b/V) was added to this solution. After mixing for 20–30 min, this solution was allowed to elapse in order to make the solution bubble-free. The mixture was passed through a peristaltic pump and allowed to fall dropwise at 60 drops/min into 50 mL of 0.1 M calcium chloride. In order to get isoniazid: alginate ratio of 60:40, 6 mL of 1% isoniazid was added to 2% alginate solution (composition ratio isoniazid: calcium alginate of 90:10; 80:20; 70:30; 60:40; 50:50 and 40:60; 30:70; 20:80 and 10:90 as formula code A-I), respectively. The beads were formed immediately and were left as such for 24 h at room temperature. Subsequently, the beads were recovered by filtration, washed with distilled water, and dried at room temperature.

Coated isoniazid-calcium alginate with chitosan

The isoniazid-coated calcium alginate was soaked in chitosan solution. The concentration of chitosan was varied 0.1-0.5% (w/v) and the time of soaked is 10 min, after being filtered and dried at room temperature for 24 h, produces isoniazid microparticles. To prepare 0.1% (w/v) chitosan solutions, 100 mg of chitosan dissolved with 100 mL of acetic acid 1M solution.

I attached the related article for you.

To remove the bubble - you can use sonication or centrifuge for a few secs. In general, to prepare a blend solution - I mix both polymer (solid) together and then add DI water/PBS and then allow to dissolve it on an orbital shaker overnight. The next day would centrifuge for a few secs to remove any bubble. Hope this helps.

This article might be useful

https://www.nature.com/articles/s41598-020-57666-8

https://pubs.rsc.org/en/content/articlelanding/2013/tb/c2tb00126h

Ms. Sara Sheikhlary & Mr. Sourav Roy thank you for the references It is very helpful for both lab protocols plus writing publications.

Dear Forbes Avila ,

You're welcome, hope you get the results you expect and wish you success in your projects.

Mr. Ajeesh Chandrasekharan thank you for your suggestion. I have tried using a slow but steady approach (low rpm over a long period of time) instead of high RPM over a short period of time. The bubble formed was minimum than I settled the solution overnight. The solution is uniform and no bubble was formed.