Hi everyone,

I normally use sep pak C18 cartridges to separate my molecule of interests from copper. I successfully used cartridges with 360mg sorbent weight and I recently bought the cartridges from same supplier with 5g of sorbent to scale up the purification. I applied the same protocol with same elution gradients and I couldn't obtain my product, it wasn't eluted in any of the gradients that I used. Do you have any suggestions on why this happened? How does the increase in sorbent weight affect separation?

Thank you in advance!

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