01 January 1970 3 5K Report

The first step of intracellular flow cytometry is fixation of the cells using formaldehyde. What is the reason behind this process ???

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DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

ANSYS APDL RC Beam modelling?

Hello I have modelled RC beam in ANSYS apdl. first create volume, then create keypoints & create a line joining that keypoints to make reinforcement. I am working on thermal analysis, for...

20 January 2021 7,344 2 View

ANSYS APDL tabular entry related?

Hello I want to add thermal convection load on specific area of concrete volume. it asks for film coefficient which is a constant value but the bulk temperature has no. of values that i have as...

18 January 2021 6,627 1 View

What is the best way to maintain constant concentration of a volatile organic liquid in an open experimental set-up?

The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.

27 December 2020 7,325 2 View

How i can input temperature depedent stress strain curve for concrete & steel in ansys?

i am working on thermo-mechanical analysis of RC beam using ANSYS. i want to input temperature dependant stress strain curve in ansys. i tried using elastic > multilinear but a warning attached...

23 December 2020 9,928 2 View

What are some best features that can be added in IoT based pollution monitoring system ?

Actually I am developing IoT based pollution monitoring system and need some ideas which help in developing great product with great features

10 October 2020 5,657 7 View

Can LoRa Technology be used for managing underwater data center ?

As per the latest research, Microsoft finds underwater data centers are reliable, practical and use energy sustainably so how can we enable IoT with LoRa technology in the underwater data center...

05 October 2020 535 4 View

How can I calculate the concentration of nanoparticles being used in UV-visible absorbance scan?

I have taken 1000 PPM of nanoparticles in 1 ml of RO water and pursuing its UV visible spectro absorbance scan. They are showing respective peaks on the baseis of energy transition of electrons...

19 September 2020 6,276 3 View

How can Gold be extracted from mining concentrates?

I work at a precious metal refinery. We extract gold using aqua regia. However, sometimes Gold does not dissolve in AR. Sometimes it precipitates due to cold weather? Is there a way to make sure...

07 August 2020 7,059 3 View

Chemistry behind synthesis of boron nanoparticle?

Hello everyone, I want to know whole chemistry behind synthesis of boron nanoparticle via chemical and biogenic methods. Focussing on why naked boron is not used as I have seen in papers, boron...

05 August 2020 1,195 14 View

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What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

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What could be some reasons why unstimulated monocytes are getting activated?

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28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

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