Cells fixation with formaldehyde.

More Sourbh Garg's questions See All

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How to select a remapping technique for different type of variables?

I am working with temperature, precipitation data (0.1 degree from CDS store) and population density data (2.5 min from SEDAC) and often have to change the resolution of either data to achieve a...

29 May 2024 9,650 2 View

How do I solve the "gmx anaelig command facing Segmentation fault (core dumped)" error in gromacs?

I gave this command - gmx anaelig -v eigenvectors.trr -f md_0_10_center.xtc -s md_0_10.tpr -n index.ndx -comp comp.xvg -2d 2d.xvg -b 34 -tu ns -first 1 -last 2

27 January 2024 1,775 2 View

Which Polarisation is better?

Polarisation in Piezoelectrics The more the better or the less the better?

12 November 2023 8,099 0 View

What statistical approach is used for paired sample study design in MetaboAnalyst?

For unpaired sample test, t-test or ANOVA is used to compare differences between groups. What statistical approach is used for paired samples in MetaboAnalyst?

15 September 2023 8,567 1 View

I got larger particle size using TEM than DLS, although there is no aggregation as shown in TEM image then what could be the reason behind it?

Particle size by tem found to be 350 nm while dls give size of 200 nm, what could be possible reason?

06 August 2023 9,018 6 View

Which is a better hysteresis loop?

Hey All!! While performing the comparative analysis of different piezoelectric materials, I landed on a situation to compare the Polarization Strength and Electrostrictive Strain Tensor...

17 July 2023 9,314 0 View

Which is a better hysteresis loop?

Hey all! I am trying to compare different materials (piezoelectric material oxides), to understand their polarisation (vs Electric Field) and Electrostrictive Strain Tensor (vs Electric Field)...

16 July 2023 6,230 0 View

Which is a better loop?

While performing the comparative analysis of different piezoelectric materials to study their applications in the biomedical sector, I came across the following graph. The hysteresis loop in the...

11 July 2023 8,207 2 View

Which is a better hysteresis loop?

While performing the comparative analysis of different piezoelectric materials for biomedical applications, I came across the following hysteresis loop. This loop represents two different...

11 July 2023 241 3 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Ankit Awasthi

Will you elaborate I am not familiar with this

Sourbh Garg

Today I read on google that, The first step of intracellular flow cytometry is the fixation of cells with formaldehyde. So, I am eager to know about the actual process mean, what makes the formaldehyde to fix the cells ???

Jitendra Kumar Shandilya

You will find explaination for all the fixative used in flowcytometry.

https://bitesizebio.com/22141/fixation-and-flow-cytometry/