We will be conducting Insecticide resistance surveillance on Philippine mosquitoes. I just wanted to know if it is acceptable to use the CDC Bottle Assay for our study even though we are in the Philippines. Will there be a problem in terms of the acceptability of the results to the reviewers if we use the CDC Bottle than the WHO?
I am hoping we can be clarified on this matter.
Thank you!
WHO and CDC bioassays give similar results with regards to the susceptibility of mosquitoes to insecticides
Comparison of the standard WHO susceptibility tests and the CDC bottle bioassay for the determination of insecticide susceptibility in malaria vectors and their correlation with biochemical and molecular biology assays in Benin, West Africa | Parasites & Vectors | Full Text (biomedcentral.com)
In terms of which method is acceptable, WHO recognises the use of the CDC bottle bioassay method. Indeed, WHO published the document 'Determining discriminating concentrations of insecticides for monitoring resistance in mosquitoes: report of a multi-centre laboratory study and WHO expert consultations' (https://www.who.int/publications-detail-redirect/9789240045200), which includes the bottle bioassay method.
As to which method is more appropriate, this depends very much on the insecticide you want to use for susceptibility monitoring. For insecticides which are suited to use in the WHO tube bioassay format, WHO's collaborating centre at Universiti Saina Malaysia produces impregnated papers. For other insecticides (e.g. chlorfenapyr and clothianidin), the bottle bioassay is the preferred method. For this, you will of course need to obtain all of the required materials, including technical grade insecticides and, where appropriate, an insecticide adjuvant.
Thank you for all of your answers. Much appreciate Dr. Kapoor and Dr. Small
The CDC bottle bioassay is designed to determine if a particular formulation (combination of the active ingredient in the insecticide and inactive ingredients) is able to kill an insect vector, such as a mosquito, at a specific location at a given time. It can detect resistance to insecticides in mosquitoes and other insects. The technique is simple, rapid, and economical, compared with alternatives. Bioassay results can help guide the choice of insecticide used for spraying.
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