Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

STS Score - Excel sheet calculator?

I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?

25 January 2021 958 4 View

Correction of co-author information?

Need to change Hossam Kandil picture and affiliation Hossam Kandil Professor of Oral maxillofacial radiology, Cairo University Not Cardiology This is wrong Kindly help me correct the...

02 August 2020 5,203 5 View

What has been going on regarding blood transfusion and heart transplants despite Covid-19?

Heart transplant and blood transfusions are very sensitive whether or not there be any pandemics. How do you think is the pandemic affecting these and how could this be managed, like how blood...

28 May 2020 3,283 9 View

Does a bicuspid valve confer any advantage over a tricuspid valve in terms of fluid dynamics in a human heart?

I've been grappling with this question for a very long time. There must be a logical reason to explain why almost all humans have one bicuspid mitral valve and 3 tricuspid valves in their hearts....

01 February 2020 2,644 1 View

Cardiac rupture following electrocution in birds: is it common?

24 November 2019 9,676 2 View

Are their standardized exercise guidelines and methods for individualization of exercise dose for patients with cancer?

22 November 2019 3,310 9 View

Are their standardized exercise guidelines and methods for individualization of exercise dose for patients with cancer?

10 November 2019 3,148 6 View

Where can i find PCG(Phonocardiogram) with ECG(Electrocardiogram) database?

I found some ECG along with PCG from Physionet 2016 challenge dataset with name "a". But, description of ECG is unknown hence i am unable to apply particular algorithm to detect important...

24 December 2018 1,017 5 View

How to cite tables and figures?

Hi. Do we need to ask permission from the related publishers if we want to use tables and figures from journal article or a citation is enough ?

11 December 2018 4,899 6 View

Where can i find PCG(Phonocardiogram) with ECG(Electrocardiogram) database?

I found some ECG along with PCG from Physionet 2016 challenge dataset with name "a". But, description of ECG is unknown hence i am unable to apply particular algorithm to detect important...

11 December 2018 923 3 View