Best analysis for this longitudinal study?

Hi everyone, I need help for what analysis is better for this longitudinal study. In my study, I measured the attachment security of children at 5 time points. My dubts are that: 1) spacing...

07 June 2020 9,834 2 View

Could you suggest a microplate reader for bacterial kinetics?

I would like to purchase a microplate reader to assay bacterial growth kinetics when exposed to different conditions (i.e.:salt concentration, different sugars, etc.). Ideally, I am looking for an...

11 September 2019 3,129 2 View

Can anyone share a good protocol for coating plates/dishes/coverslips for mouse hematopoietic stem cells?

I am currently facing problems with my cell attachment. Can anyone help me suggesting a protocol for coating plates/dishes/coverslips with Poly-L-Lysine for hematopoietic stem cells? Would you...

12 November 2018 6,693 6 View

Did someone use an antibody against caspase-9 for immunoprecipitation from mouse cell lysates?

14 December 2015 9,546 2 View

Can anyone suggest some good laboratories in Northern Italy for standard environmental testing?

I need to identify some good Labs placed in Northern Italy to be able to make vibrations, thermal cycling, ingress protection, salt spray and cold soak tests for automotive application (and not...

11 November 2015 5,518 5 View

Who are the worldwide experts/opinion leaders in primary cancer cells 3D culture?

25 October 2015 1,063 3 View

Does Someone know if FITC is soluble in chloroform?

I know it is soluble in ethanol, acetone, DMSO, DMF; someone knows about chloroform?

19 March 2015 4,740 2 View

Why don't my EBV transformed B cells secrete antibody?

Dear all, I transformed with EBV B cells from total PBMC and after 1 month I isolated IgG+ cells using Miltenyi beads. Keeping in culture these cells with CpG and without, I got proliferation but...

16 March 2015 9,839 5 View

Does anyone know which primers should I use to genotype CSHL gene trap lines?

I am trying to genotype some CSHL gene trap lines in A.thaliana and I am having some difficulties in designing the primers. For those on the flanking regions (ie, the LP and RP), I used the iSct...

12 February 2015 2,011 3 View

Fluorescein stabilization in ethanol solution for temperature measurements

I'm working since several months with a solution of Fluorescein Disodium (C20H10Na2O5) and Ethanol. So far, the spectrum of this solution is changing due to unknown parameters and I would like to...

01 January 1970 7,629 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Do you have papers on hindlimbs myology of birds to share?

Hi all! My work team and I are looking for papers on muscular descriptions of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than...

22 February 2021 8,330 6 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

How to Measure Consumer Willingness To Change from petroleum-fueled vehicle to electric vehicle?

I wanted to calculate the probability of one consumer shifting from using a petroleum-fueled vehicle to an electric vehicle. Since my background is in engineering I do not know much about how to...

15 February 2021 9,190 5 View