Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests?

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests? I am looking for carbon storage potential ie carbon biomass for a number of native trees and shrubs...

08 July 2020 5,236 2 View

Issues with Image J Thresholding?

I am wondering if anyone has had issues with their image J thresholding? I am trying to perform colour deconvolution, but when I convert the image to binary, I am unable adjust the threshold to...

01 April 2020 3,812 3 View

Where can I get survey data to compare the US to subSaharan Africa, China on the Impact of Climate Change?

Preparing a research paper. Other countries/geographies could be substituted

12 March 2020 195 4 View

Quantifying ADC values from a ROI?

I currently have both DWI and ADC sequences however I want to calculate the mean ADC value for a defined ROI (ischemic stroke lesion). I currently use ITK-SNAP, Image J and Horos for viewing...

03 March 2020 9,749 4 View

Which software is good for analysis of band size in gel image? How can we analyze the curve band image?

Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder.

17 February 2020 7,762 5 View

How do I align a sequence set to one reference sequence?

I am currently trying to find homologues of a protein I am working with, but BLAST has been giving me nothing useable. I have now found a dataset of 1500 protein sequences of potential candidates...

26 June 2019 5,596 2 View

I need an analytical balance that weighs organic samples at 0.0001 g for stable isotope analysis. What models are reliable and cost-effective?

For weighing stable isotope samples

18 October 2018 687 4 View

Assuming you are teaching a class and a student ask you a question you cannot readily answer. What would you do?

When you find yourself in this situation, what would you do intelligently so your students don't believe you can't answer?

02 March 2018 818 62 View

How can drug abuse be mitigated among students?

There is this canker of students abusing drugs in school. How can this be controlled?

01 March 2018 8,003 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

How to assign two different material/section properties in Abaqus(UMAT)?

Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...

11 February 2021 9,607 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

How to write cyclic loading in OpenSees?

Hi there, I'm having trouble with adding cyclic loading in OpenSees software. My task is to model a reinforcing bar under cyclic loading and compare the response with the experimental results....

04 February 2021 8,158 1 View