Can you validate SFC?

More A.M. Sharaf's questions See All

Hi everyone ... Why contamination with anaerobic bacteria, yeasts and molds increase (not decrease) the pH of the culture medium?

Thanks in advance

17 March 2024 802 3 View

I need advice because I am writing a book- Book Title: "Zero Cash Trading: Navigating Away from Paper Currency Outside the Saudi Banking System"?

**Table of Contents:** **Chapter 1: Introduction** 1.1 Introduction to Trading Outside the Banking System 1.2 Importance of Transitioning to a Digital System **Chapter 2: Financial Context in the...

13 December 2023 7,165 0 View

How to calculate the neutron flux using Geant4?

Is there any free software for caring out these calculations.

21 October 2023 4,961 0 View

I want to do cellulose,hemi cellulose ,pectine and lignin extraction from pineapple fruit flesh. Which methodology will be the best?

this will be done to knoe fibre composition

19 September 2023 8,199 2 View

I need a protocol to reduce any oxidized (Fe3+) hemoglobin in my sample to (Fe2+)?

Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....

13 September 2023 9,133 4 View

Does anyone know the equilibrium constant of guanidium chloride to urea and then of urea to isocyanic acid?

Hello, I need to use guanidium chloride to denature a protein. However, I’m reluctant because it is absolutely crucial for my experiment that no isocyanic acid is formed. I need to know the...

13 September 2023 4,587 1 View

Hı!, I'm estimating a Panel VAR using the stat pvar package which estimates homogenous pvar. How can I get irf for individual country as well thanks?

Please suggest any other method for estimating Heterogenous Panel Vector AutoRegressive thus allowing individual irf to be computed.

12 June 2023 9,061 3 View

Can i use probenecid (water insoluble) in measuring intracellular calcium concentration from HIT_T15 cells?

Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...

17 April 2023 6,450 0 View

What is exon-exon junction?

Good day, The presence of a premature stop codon more than 50–55 nucleotides upstream of the final exon–exon junction is a strong predictor of NMD. Kindly, how many nucleotides included in the...

13 March 2023 5,649 1 View

Can I transfare the cells from well to another well?

I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates. my question is can I transfer my cells to 96 well by using trypsin is that possible?

12 February 2023 7,826 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

Hamid Reza Baghaee

Dear Professor Sharaf

I sent a request for contribution in this chapter book to your email. Would you please keep me informed about your opinion?

Regards,

A.M. Sharaf

PLease see my email.

Ahmed M. M. Rashad

Dear Prof/ A.M. Sharaf

It is my pleasure and honor to me. my mail is

[email protected]

A. Rashad

Thank you.

We will. Start with facts applications in Renewable Energy and micro grids as well as V2H/ V2 GG battery charging schemes.