Can you use the Radio isotopic labeling method to study the mode of action of the pesticide in the insect?

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High Power control for 48V battery pack - High current control?

Hi, I am working on 48V Lithium-Ion battery pack. I am wondering if there is fast MOSFET (or any other technology), to control the current of charging and discharging the battery with high...

20 January 2021 1,717 4 View

GROMACS: Cannot rename checkpoint file; maybe you are out of disk space?

Hi! i am running a simulation using a cluster and I have encounter an error during my nvt equilibration like this: Cannot rename checkpoint file; maybe you are out of disk space? I deleted some...

03 January 2021 3,544 2 View

How can the resistance of paving asphalt be increased to acid rain?

Modification of the rheological properties of asphalt

25 December 2020 7,390 2 View

Can someone please share the matlab code of FBMC/OQAM PAPR reduction with TR and PTS methods?

I am trying to work on reducing the PAPR of the FBMC/OQAM with the TR and PTS methods. for that if someone can share with me one of these matlab codes. my e-mail: [email protected]

19 December 2020 7,764 1 View

Appreciate the quality qualities of cheese ?

How to estimate the acidity of a cheese?

13 December 2020 4,890 4 View

What is the appropriate polymerase to amplify gDNA greater than 10kb?

I am going to clone gDNA fragment > 10 kb into a pCR TOPO vector. I performed the PCR using KOD PLUS Neo polymerase for 3-steps PCR reaction, I am not able to find the specific size. Several an...

03 December 2020 1,939 5 View

How to get the data of the changing the distance between two Pionts with the applied force?

does anybody knows how can i get the change in the distance between the notch nodes (two points showing in the figure ) vs the applied force in every step as a table out put or curve in...

23 November 2020 352 3 View

Why I got low CI in the EBSD?

Hi I got a low confidence index in the EBSED analysis even after days of polishing using 1 to 0.02 um suspension using 1 lb force, the sample was Al/W composite (has intermetsllic interface)...

26 August 2020 3,547 4 View

Hi Is it needed to etch a sample in order to see dislocations? SEM or TEM microscope should be used for that?

hi please I have Al/W sample and I am looking for screw dislocations on the intermetallic surface, my question is: Should the sample be etched? and should I use the SEM or TEM microscopy? what...

06 August 2020 9,703 2 View

How can i increase my validation and test accuracy?

I am using google colab to train a model for sign language recognition using this dataset: https://www.kaggle.com/feronial/turkish-sign-languagefinger-spelling I tried different architecture...

08 May 2020 4,098 5 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View