How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

Failed Chromatograms on HPLC-ESI Orbitrap?

Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...

09 February 2021 3,595 8 View

Do you know any available open-source data on animal behavior for model validation?

In my research related to monitoring the cow behavior with help of sensors I have a permanent and principal problem with the data for model validation. I tried to find some available open-source...

26 December 2020 8,197 2 View

Do you know of research in communication/media studies using "Activity theory" ?

Hello, For a project which studies how teens deal with social media and their interfaces, I'm interested in so-called "activity theory" (also know as cultural-historical activity theory). I know...

21 October 2020 2,364 4 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Did somebody tried to use the attR1 and attR2 sites without the chloranphenicol resistance gene i.e. AttR1-ccdB-AttR2?

I am trying to design a vector as compact as posible to test DNA transcriptional enhancer and/or repressor activity, I already have a compact bacbone (pICOz vector), minimal promoter, translation...

22 August 2020 1,083 1 View

How to amplify phage DNA (dsDNA) for producing restriction profiles?

Hi everybody! I need to amplify phage DNA so as to be able to produce restriction profiles using different restriction endonucleases. Can anyone recommend which primers should I use? I know random...

06 August 2020 1,194 3 View

Is there any method for tuning Subspace Predictive Controllers (SPC)?

My current research is about SPC and I'm interested in tuning strategies for these types of predictive control approaches. The cost function is on the photo and the tuning parameters are Np...

23 July 2020 1,176 1 View

Some TWIP steels are susceptible to hydrogen-induced failure, but adding up to 2% aluminu improves the situation. What mechanisms explain this phenom?

On the role of aluminum in TWIP steels

22 July 2020 5,670 4 View

Do you think the teacher should switch roles with the students while teaching a foreign language through TIC?

There are some theories, as Pelgrum and Law (2003) suggested that it is necessary to make a switch between the teacher and the student roles. I mean, the student should be also the active role in...

15 July 2020 5,169 15 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View