We are going to investigate the effects of some Iran's medicinal plants and its phytochemical compounds on cell cytotoxicity and discovering new anticancer drugs. Would you please express your valuable comments about this study in order to improving our project.
There is a correlation between cell toxicity and Artemia salina lethality assay; this may be a good starting point.
The is many such protocol available in literature. First try to identify the cell line in which you are wishing to work.
Reading through materials and methods from a number of papers describing cytotoxicity assays could be the way forward
To begin with, carry on a SRB assay with at least six different human cancer cell lines. If you get anticancer activity in at least four of them, then you should proceed for further mechanistic studies. Later you can perform MTT which is also widely used for these purpose.
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