Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

HowScholarships, Fellowships and grants in Materials engineering for international students to study abroad?

On Line student doctorant looking for first Master PhD student doctorant as UNICAF now in Materials engineering for international students to study abroad on Line and To Bricks LTGS and To have...

10 December 2020 3,686 3 View

Merging two same papers in a one paper?

Hello, I have three same papers which have different numbers of citations and different number of readings and I would like to merge these three papers in one paper with taking account of a new...

20 September 2020 5,275 6 View

Does polysome profiling in 4mL tube instead of 12 mL needs a scale down in ultracentrifugation time ?

Hello everyone, I recently started several polysome profiling experiment and something bugs me out. Every protocols I found use 12mL tube. They load their lysate containing ribosomes attached to...

19 September 2020 8,717 10 View

What is the sensitivity of SHM technique based on electromechanical impedance method to identify a crack in a concrete beam?

The sensitivity of the technique is important to know how to identify the appearance of cracks

17 August 2020 4,156 9 View

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31 May 2020 6,077 6 View

How can you efficiently collect a ground-nesting bee when it is inside its nest?

I am looking for an efficient way to collect a ground-nesting bee at its nest, so that when encountering a ground-nesting bee nest, I could determine what species created that nest. Ideally, I was...

21 February 2020 5,269 3 View

Does someone has an interest for XIXth century herbaria prepared in Brazil?

While investigating about French women botanists in the XIXth Century I chanced upon two herbarias, made from plants collected in Brazil. I am looking for someone who could help to evaluate the...

07 October 2019 2,560 6 View

Journals to submit a method to assess Vulnerability of Hotels to Earthquakes ?

Dear all, What are the most appropriate journals to submit a method we developed to estimate the seismic vulnerability index for touristic accommodations ? and its application to a pilot zone of...

08 September 2019 8,878 3 View

The "4p1000" initiative on soil carbon storage: Should the name be changed?

At the COP 21, in France, a few years back, an initiative was proposed to increase the carbon content of soils by 0.4% or 4 per 1000 per year. The claim was made that, in so doing, soils could...

28 May 2019 3,080 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View