I am looking for unconjugated primary antibodies against CD3,CD4,CD8,CD11c, CD14, CD21,IgM, MHC-II in Pig FFPE tissue samples. Has anyone used Pig FFPE tissues for IHC against these markers ?
Hi Shaurav, Just to start you on your searches: I have found a mouse anti-CD3 antibody [CD3-12] which works on FFPE porcine material. It is sold by GeneTex, and it seems that the same clone is sold by Abcam. GeneTex has a convincing microphotograph and both sellers quote publications - worth checking. Both sites have also rabbit antibodies to CD3 working on fixedporcine material but no literature or microphotographs.
I haven't seen a reliable anti-CD4 antibody, found one but the microphotographs were far from convincing.
Good luck with your project.
CD3 antibodies are generally cross-reactive across most species, the 3-12 clone mentioned above should work on pigs. Beyond that it's probably going to be difficult, since there's not a lot of work done on pigs so commercial antibodies probably haven't been tested on them. You can try a search on Biocompare specifically for pig reagents, or you can look for antibodies for these markers that are noted to be cross-reactive across rodent, dog and human, in that case it is likely they should work on pigs.
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