Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

Are you a virtual team member/leader who would like to participate in a Delphi study on International Online Collaboration Competencies (IOCC)?

You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...

11 November 2020 9,715 1 View

Bonferroni or Tukey: Which post-hoc test is adequate for my data?

Hello, I am running an ANOVA between 3 groups (Group 1 N=148, Group 2 N=147, Group 3- N=144; equal variances assumption met) . I am having difficulties in deciding upon the most adequate post-hoc...

21 October 2020 2,182 5 View

Does anyone know a good paper about deep watershed application for XCT images of rock?

I was able to find some applications of deep watershed for image instance segmentation, but nothing for rocks. Could you please recommend any study?

07 September 2020 5,731 8 View

What are reaction conditions for aromatic sulfonation of a dibenzyl ether derivative?

I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...

04 August 2020 4,446 7 View

Can you recommend any publications or authors on ethical and eco-friendly marketplaces?

Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon

27 July 2020 7,302 3 View

What are your nanoindentation tips cleaning tricks?

Dear users of nanoindenters, I was wondering what procedures do you usually use to clean your diamond nanoindentation tips? I know that there is nothing specifically described but some internal...

08 July 2020 5,926 5 View

How good is the quality of spectra taken by portable NMR spectrometer?

At the moment our laboratory would like to buy portable NMR spectrometer and we would like to see some real not promo spectrograms. Could those who constantly work with such devices share spectra...

22 June 2020 8,144 6 View

What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method?

What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method? Can the EEG record accurately activity in deeper brain structures...

11 March 2020 1,087 6 View

Online testing solutions: any online tool permitting recording of participants' verbal responses?

Covid-19, apart from all health consequences, is also having an impact on research projects with human participants. Universities and schools are shutting down and, even when labs are still open,...

11 March 2020 2,711 16 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

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01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View