Add Acetic acid D4

Add Acetic acid D4

agreed with Andrei Blasko, chitosan is readily soluble in weakly acidic media - anything below pH 6 will give you the ammonium form - usually ammonium carboxylates are prepared for water solubility (acetates, formates would be best suited for NMR - for other purposes such as formulation glutamates, lactates, succinates etc. can be used too.)

Better first preparing your dilute deuterated acidic D2O solution carefully then solubilizing chitosan (to avoid accidental hydrolytic cleavage if you accidentally make it too acidic)

Thank you, Andrei Blasko and Cedric Lion, your information obviously will help me.

Regards

Vikash Chandra Roy I do use this method below to calculate DDA:

Article Various Methods for Determination of the Degree of N-Acetyla...