Can you suggest how to quantify extracellular markers in immunohistochemistry slides?

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Elisa Avolio

Hi Rocco,

in the past I quantified the amount of a protein released in the extracellular matrix in myocardial tissues. I performed an immunohistochemical staining using the system DAB-peroxidase to reveal my protein. Then I measured the positive area (using the ImageJ free software) and I related it to the total area of the tissue section. So you obtain the fraction of tissue positive for your marker.

I think this is the best solution to quantify extracellular markers.

Elisa

Rocco Cappellesso

Thank you Elisa. Did you publish that work? Could you send me the reference?

this is the work: "Role for substance p-based nociceptive signaling in progenitor cell activation and angiogenesis during ischemia in mice and in human subjects", Circulation 2012.