Can you recommend an article, on lipid to carbohydrate ratios in isoenergetic feeds for fish?

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Why is my protein eluting in the salt wash when applying IMAC?

Hi everyone! I currently have the issue that my proteins are all eluting at the salt wash that I apply during my IMAC (HisTrap 5ml HP column). My proteins are all His-SUMO-tagged. They are...

25 May 2024 8,144 3 View

How do regions of injection locking of three coupled nonlinear oscillators look like in a devil's staircase in 3D (or 2D plot of the rotation number)?

By a 3D devil's staircase I mean the plot of the rotation number (or any invariant property distinguishing states of the system) as a function of the ratios w2:w1 and w3:w1, where w1, w2 and w3...

26 June 2023 357 0 View

What is minimal time of hybridization for FISH?

Hello! I am curious about the minimum hybridization time for the custom locus-specific probes for FISH. I know that it should take at least 12 hours at 37 degrees Celsius, but is there an even...

31 May 2023 9,886 2 View

Building a VECM model with all I(1) variables but the information criteria (AIC and SC) show weird results for lag selection?

Hello, I am building a VAR modell in order to discover how (WTI) oil price shocks (changes in the WTI price) and 3 macroeconomic control variables (gdp_growth, Log-Interest rate, Log-exchange...

21 November 2022 1,157 19 View

Why is the spin-orbit splitting of Si not visible in the Si2p XPS specrtum of SiO?

XPS spectra of the Si2p core level usually show the spin-orbit splitting of this level (1/2 and 3/2). In the case of SiO_x, all spectra I have found show a particulary narrow component, which...

29 August 2022 3,305 4 View

Aluminum evaporation results in blue and dull top surface?

Dear all, The last weeks I'm struggling with the thermal evaporation of aluminum. We're running the evaporator inside a nitrogen filled glovebox. I'm evaporating the aluminum with a pressure of...

13 April 2022 8,286 15 View

Is there a current research project that deals with the application of assemblage thinking on the socio-economic performance of medium-sized towns?

As I know, so far there has't been the attempt to combine those issues, but I'm not sure. Could be an interesting research item for urban studies

23 March 2022 2,671 6 View

Serum-free medium or medium sublemented with serum for Sf9 protein expression? What are the advantages? Is any BEVS superior to the other?

Hi everybody! I am currently facing a very nasty protein that I am trying to purify. Besides the need for several purification steps, the lack of proper expression in insect cells gives me a hard...

02 February 2022 4,157 0 View

LPA: Which recommendations for BLRT (TECH14)? / Treating Likert Items as interval or ordinal scaled variables ?

Dear Ladies and Gentlemen, I am currently working as a Master's student on several publications that methodically rely on latent profile analyses. In the context of these LPAs, I have repeatedly...

04 January 2022 1,194 4 View

Does anyone know a scholar with knowledge on French in Congo-Brazzaville?

For our manual on Romance languages in Africa (RoLA) at the University of Passau (Germany), we are looking for an expert on the variety of French spoken in Congo-Brazzaville who could contribute a...

08 December 2021 9,829 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

Why does total carboydrate content decrease when increasing the vacuum degree?

I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...

13 July 2024 592 1 View

Phospholipids storage conditions?

What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...

27 June 2024 2,138 2 View

What is the best way to calculate the weight of each lipid component needed to achieve the desired molar ratios?

I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...

26 June 2024 1,135 0 View

How can i use eastern blot for fisrt time to study nucleolar lipids an PA in mitosis?

Hi everyone It is my first time working with this technology, so someone here can give me your experience my objective is to study different nucleolar lipids and their interaction with...

25 June 2024 7,351 0 View

Why does a line appear in the middle of the carbohydrates TLC plate?

The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...

24 June 2024 9,829 3 View

Lipid composition of the aortic membrane cells (smooth muscles)?

Hello, do you know what the phospholipids of the membrane of rat smooth muscle aortas are composed of? outer membrane-inner membrane

19 June 2024 221 0 View