25 October 2017 3 3K Report

I currently work on a project to evalute the alamar blue assay for testing cytoxcity of allicin on MCF-7 and LS-174T cellllinie . I couldn't find the information how to subculture 3D culture cells.

Any help would be greatly appreciated :)

More Michael Ruegenberg's questions See All
Has anyone else received an email regarding payment for citation?

I recently received this email message: "Dear Colleague, I trust you are well! I am writing to propose a mutually beneficial collaboration. I have many research papers that need citations. If...

27 June 2024 9,627 1 View

Do you have a protocol for stimulation of mouse B cells by PWM?

Looking for a protocol for the stimulation of B cells with pokeween mitogen

22 June 2024 4,882 4 View

Which standards, frameworks or best practices for AI governance do you know?

Frameworks for AI governance The use of artificial intelligence in the company requires its control and monitoring by the company's governing bodies due to legal and regulatory requirements....

17 June 2024 8,956 5 View

Can someone explain the intuition behind, Blue stars are small, but wavelength is big. Red stars are Big but their wavelength is small?

can someone use quantum mechanics to explain the intuition behind this phenomenon?

07 June 2024 9,584 2 View

Can delirium cause brain damage that is similar to damage caused by a stroke?

Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...

07 June 2024 8,723 2 View

How does one get and add a DOI to an existing file upload?

to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?

06 June 2024 5,095 1 View

Which form/type of epigenetic modification is most closely associated with transgenerational effects?

Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...

31 May 2024 4,613 3 View

Help! Alizarin Red not staining?

I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...

28 May 2024 4,068 2 View

How can i get available datasets for Kaduna metropolis at least 2 busy 4-way intersections for research purpose?

I am working on vehicle pedestrian safety at 4-way intersections with traffic signal control lights. I need dataset available for Kaduna metropolis busy 4-way intersection with pedestrian count...

21 May 2024 4,872 1 View

What is the rate of acceptance for Business and Professional Communication Quarterly?

Where can I find Business and Professional Communication Quarterly's manuscript acceptance rate?

18 May 2024 1,303 1 View

Similar questions and discussions
Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close