can you please tell me the compostion of red blood cell lysis buffer for rna extraction from blood ? im using Geneaid rna extraction kit.?
First of all, I would suggest that if you are going for RNA extraction, use only fresh sample of blood. Do not use blood which has previously been frozen and thawed or the sample which has been used for any of the DNA application.
You could use ammonium chloride lysis buffer. The composition here is for 10X in 100mL miliQ to be stored at 4 degrees.
NH4Cl (ammonium chloride) - 8.02gm
NaHCO3 (sodium bicarbonate) - 0.84gm
EDTA (disodium) - 0.37gm
Hallo.
We use the following lyses buffer for RNA as well as DNA extraction from fresh blood:
NaCl 150mM
EDTA pH8 2mM
NP40 1%
Tris HCl 50 mm pH 7.5
Good luck
thanks alot for answering my question ,im very greatful for you
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