Hello,

you can find attached a photo where colonies of both, wt strain and ko mutant (mut), are shown. A gene encoding a key protein probably involved in CPS synthesis was deleted by double crossover.

They are at the same concentration, but the mutant did not present the typical heterogenous phenotype of the strain, since no transparent colonies were seen.

Any suggestion about the function of the lacking gene? I would appreciate any help because this is not my field.

Thanks!