I have obtained by ultracentrifugation a band of HDL, mainly HDL3 characteristics, with a protein concentration of 10g / l. Can anyone advise me on the steps of how to identify these proteins?
I found one old article where authors characterized a human HDL by gradient gel electrophoresis. I believe this is a very old method and nowadays more advanced proteomics based characterization strategies are available. I am sharing a combined pdf file for the old paper as well as a very recent articles employing proteomics approach which I think will be great resources to begin with.
Hope that helps.
Some strategie for characterization could be the chemical and protein composition and the size distribution. You can try this approach with the methodology described in this paper. There is also a lot of methodologys for to analyze their functions as antioaxidant, at the cholesterol metabolism an more. Is up to what you are more interested on the papers that you could read.
Decreased activity of lecithin:cholesterol acyltransferase and hepatic lipase in chronic hypothyroid rats: Implications for reverse cholesterol transport. Molecular and Cellular Biochemistry 246: 51–56, 2003.
Palmitic acid in HDL is associated to low apo A-I fractional catabolic rates in vivo. Clinica Chimica Acta 378 (2007) 53 –58
Enzymatic assessment of cholesterol on electrophoresis gels for estimating HDL size distribution and plasma concentrations of HDL subclasses. Journal of Lipid Research Volume 51, 2010
Good luck
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