What are the chances of review paper getting published by an independent researcher?

I have written a review paper centering on the ongoing COVID-19 pandemic and it has been communicated for publication purposes. While the journals I am aiming for welcome unsolicited reviews, the...

03 August 2020 4,793 3 View

Does overnight resting of PBMCs help in better detection of IFNg, TNFa and IL2?

I want to detect IFNg, TNFa/IL-2 and CD107a in NK and T cells. I am going to stimulate them with different stimuli (PMA, Anti-CD16, K562, etc). Before I start my optimization I wanted to know...

20 May 2014 806 4 View

Is there any method to determine cell cycle arrest other than using flow cytometry?

Flow cytometry is used to determine the cell cycle arrest using fluores. Is there any other method to do the same?

26 September 2013 9,406 4 View

What is the effect of amorphous impurity on XRD pattern of crystalline material?

Is it going to shift peak position and affect intensity of peak of crystalline material?

19 October 2012 6,755 11 View

Are Ct values near 38-40 suitable for gene expression analysis?

I am performing relative quantification of NOS2 gene in PBMCs isolated from human blood samples using SYBR green detection method. Ct values are in the range of 38 and 40 with very good...

01 January 1970 5,553 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View