Panc2 cell line required fro nano research?

Hi all, Does any one have pancreatic cell line- panc2. If any one have can you able to ship to India and I will provide fedex number for overnight shipment. Thank you in advance Sincerely...

06 July 2018 5,535 0 View

Reduced mitochondrial as a protection for neurodegenerative diseases?

Mimitochondrial fission is generally regarded as getting rid of bad mitochondria, perhaps the increased fission is trying to get rid of poor mitochondria and what is the purpose of reducing...

07 August 2016 1,428 4 View

Can anybody tell me what are the other bands in my immunoblotting with 6E10 antibody (covance) . See the pic for details

Alzheimer's disease

04 May 2015 5,236 10 View

Can someone help me with my transfection?

Yesterday I did transfection at 11.30AM to GSk3 beta-- cells. Usually I collect the pellet after 36 hrs which means I have to collect at 11.30PM today, but now my dishes are 100% confluent. I need...

05 June 2013 9,492 8 View

Signal in water samples when I do Real time PCR.

When I do real time PCR, sometimes I get an amplification signal in the water sample. I use AB systems Fast SYBR Green master mix (Cat NO: 4385612). I setup the experiment along with house...

01 February 2013 2,539 9 View

Measuring secretary proteins in supernatant using conditioned media.

I want to measure some secretary proteins in the supernatant and I want use conditioned media. I have proceeded in this manner: seeded the same number of cells in the same volume of medium,...

01 February 2013 1,414 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View