Can you any one suggest good antibody for detecting pTau by IHC ?

More Ramesh Kandimalla's questions See All

How to find peak period and significant wave height for a particular location in ocean?

I want to develop the figure like attached figure. How can I get, peak period and significant wave height at any given location.

13 July 2024 886 2 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View

Dear All, I have to plot a wave data similar to the attached figure in matlab. Anyone please help me in this?

How to plot like attached figure in matlab?..

25 June 2024 7,220 4 View

How to calculate drug loading and Encapsulation efficiency ?

Even after using the formula i am not getting the answer can anyone help me with one example .

28 May 2024 4,503 1 View

Black dots in cell culture ?

We have been getting this black dots in our cell culture. But it is not affecting pH of media (no color change observed) until 4 or 5 days. Cells are attaining morphology within 24hrs but once the...

12 May 2024 9,284 4 View

Increasing nozzles height of drone spryaing system increases droplet size. What could be the reason behind it? ?

03 March 2024 4,127 0 View

Which is the more accurate method to calculate LUMO value for a D-A system?

Which is the more accurate method to calculate LUMO value for a D-A system? Either it from absorption edge or from CV measurement on reduction side?

15 February 2024 5,635 1 View

How do I overcome or eliminate this noise problem during patch clamp recordings?

Hello everyone, I recently encountered a noise problem in my patch clamp experiments. I happened to observe a small current and a noise when the electrode holder was in the open circuit. The...

28 January 2024 927 3 View

How long does it take to culture single colonies from single suspension cell in a 96-well plate?

Hello! I have been culturing GM11994- B lymphocyte cells from Coriell Institute. They are suspension cells and like to grow in colonies. However, after genetic modifications, I sorted single cells...

09 January 2024 7,779 0 View

How to calculate composite cylinders vibrations?

Papers that compute natural frequencies of cylinders made of composite materials.

29 October 2023 6,669 0 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Krystyna Hincman

Hey Ramesh - Cell Signaling Technology has two great IHC antibodies for p-Tau. #15013 Phospho-Tau (Ser416) (D7U2P) Rabbit mAb

#12885 Phospho-Tau (Thr181) (D9F4G) Rabbit mAb

Ramesh Kandimalla

Hincman,

Thank you for your suggestion and I will look in to those antibodies.

Sincerely

ramesh

Russell Linscott

In addition to Cell Signaling, dianova GmbH has a lot of mAbs vs. pTau. We also list a number of other sources you may want to investigate:

http://www.linscottsdirectory.com/search/antibodies/searchStr:pTau

Click on the "More Info" in the results table to go to the suppliers' data pages. Good luck.

Thank you Russell for the information.