Please read the brochure if it working with ICC or not. if it not mentioned you must try it with serial dilution to test it.

Generally I think the answer is yes, because the preservation of epitopes of the protein you interested is comparable in intracellular flowcytometric staining and in immunocytochemistry. However, you have to try the conditions to get the best results.

Thanks Dev, Ayman and Chun. 

I tried this antibody in several dilutions from 1:20 to 1:500 but I could not receive any staining of my desired protein in confocal microscopy. The protocol for staining cells in both cases are nearly same but I don't understand why this is not working in ICC.

In theory - yes. In practice- depends. When cells a re fixed they do also change protein/epitope conformation. In ICC the cells are fixed on a slide, as I recall correctly the general technique and in cell sorting are either not fixed or post-fixed after antibody binding. Other option is that you bleach the fluorophore with the laser. The confocal photon detectors have 10-30% photon efficiency and you might just bleach with exposure to light.

Best,

Ivan

Thanks Ivan for your informative informations.