Comparing activity of crude enzyme extract (alpha glucosidase) with standard one?

I am doing experiment to produce alpha glucosidase from thermophilic bacteria. Though I can determine enzyme activity by enzyme assay but my supervisor wants me to have a positive control, in this...

08 September 2018 3,390 11 View

Hello. Can anyone please give me some help about how to determine the amount of enzyme in enzyme assay?

Hello everyone. I am doing an experiment relates to enzyme alpha glucosidase. I extracted this enzyme from 7 strains of thermophilic bacteria from a hot spring. Now I test the enzyme activity of...

03 April 2018 6,768 4 View

Hello. I need some help to calculate enzyme activity of alpha glucosidase. Can anyone give me some ideas?

Hello everyone! I have done an experiment in enzyme assay of alpha glucosidase by using pNPG ( p- nitrophenol-a-D-glucopyranoside ) as a substrate. This is my mixture reaction: 0.9ml phosphate...

31 December 2017 2,742 5 View

Production of secondary compounds in plant tissue culture.

Hello! I would love to know about the production of secondary compounds, especially about phenolic compounds which are produced in the process of culturing plant tissue. It is supposed to be...

05 June 2017 2,103 11 View

How can we isolate some bacteria strains with low population?

There are some bacteria strains that can tolerate the toxic orgainic solvent, but the number (or population) of them is very low. How can I isolate them? (The bacteria come from soil). Can anyone...

03 April 2017 3,679 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View