Phoebe Nguyen

6 Questions 16 Answers 0 Followers

Questions related from Phoebe Nguyen

Comparing activity of crude enzyme extract (alpha glucosidase) with standard one?

I am doing experiment to produce alpha glucosidase from thermophilic bacteria. Though I can determine enzyme activity by enzyme assay but my supervisor wants me to have a positive control, in this...

09 September 2018 3,463 11 View

Hello. Can anyone please give me some help about how to determine the amount of enzyme in enzyme assay?

Hello everyone. I am doing an experiment relates to enzyme alpha glucosidase. I extracted this enzyme from 7 strains of thermophilic bacteria from a hot spring. Now I test the enzyme activity of...

04 April 2018 6,827 4 View

Hello. I need some help to calculate enzyme activity of alpha glucosidase. Can anyone give me some ideas?

Hello everyone! I have done an experiment in enzyme assay of alpha glucosidase by using pNPG ( p- nitrophenol-a-D-glucopyranoside ) as a substrate. This is my mixture reaction: 0.9ml phosphate...

01 January 2018 2,800 5 View

Can we use reversed micelles method to extract alpha glucosidase?

Can we use reversed micelles method to extract alpha glucosidase? I want to extract alpha glucosidase from Saccharomyces cerevisiae and Bacillus spp. by using reversed micelles technique. But I...

10 October 2017 2,379 2 View

Production of secondary compounds in plant tissue culture.

Hello! I would love to know about the production of secondary compounds, especially about phenolic compounds which are produced in the process of culturing plant tissue. It is supposed to be...

06 June 2017 2,182 11 View

How can we isolate some bacteria strains with low population?

There are some bacteria strains that can tolerate the toxic orgainic solvent, but the number (or population) of them is very low. How can I isolate them? (The bacteria come from soil). Can anyone...

04 April 2017 3,735 4 View