Can we use quantitative gene expression levels without reference dye in qPCR MASTER MIX?

Nageswar Reddy Manchala @Nageswar-Reddy-Manchala

31 August 2018 0 9K Report

Hello

I had a small question regarding the reference dye that incorporated in qPCR master mix of 2x Syber green or Taq Man mix. Generally these reference dyes such as ROX is incorporated for the normalization. Suppose if I made a probe tagged with the same reference dye such as ROX then how to quantify gene expression level? can this ASSAY will be limited to quantitative only ?

Thanks and your thoughts are most welcome!!!

Dr. Nageswar Reddy

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