We have vaccinated pig with antigens and then, we would like to know whether we can use mice to perform a passive protection assay by using the pig immune sera. Do you know pig macrophages can recognize the Fc receptors from a pig?
I made a mistake, mouse macrophage can recognize the Fc receptors of pigs?
Hello Yung-Fu Chang,
I have no idea if there is any cross reactivity between mouse and pig Fc receptors and the respective Fc parts of immunglobolins.
But just a quick thought how you could check it:
You could isolate murine monocytes or macrophages and incubate them with some µg of pig sera. Wash the cells and incubate with an fluorescence labeled anti-pig-antibody (e.g. anti-IgG).
Run a control sample where you block the Fc-Receptor of the monocytes/mac first with an blocking antibody (anti-CD16/32 maybe?). In this sample you should have no signal, proving the specific binding of pig-IgG to murine receptors.
Measure the fluorescence signal via FACS or a plate reader.
Of note:
Firstly, It's just my idea and I'm not an expert concerning cross reactivity or immune globolins etc...
And: You might (or might not) run into some issuses if your detection antibody (fluorescence labeled anti-PigIgG) recorgnizes the same binding site of your immune globoline which would be used for the binding to FcReceptors.
Maybe this helps a little bit
best regards
Robert
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