Can we use optical profilometer to study the surface roughness of nano-sized Vesicles?

25 November 2021 1 6K Report

Is an optical profilometer used to study the surface roughness of extracellular vesicles instead of AFM?

Yes, coherence scanning microscopes can measure "roughness" in the nm-range. However, this makes only sense for very smooth surfaces and one would better call this a "waviness" measurement, because all small structures are "filtered" due to the limited wavelenght of the light. And "seperating structures" that are in the range of the difraction limit (=structures become visible in the microscope image) is one thing, calculating a useful hight value from such data is another thing.

If "nano-sized" refers to lateral dimensions, the answer is clearly no.

Badges
Science topic

More Archana Rajavel's questions See All

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Anyone can list the activities based on body schema which will enhance the nonverbal communication of mentally retarded children ?

02 July 2024 9,947 0 View

How to perform electropolymerization on the screen printed carbon electrode?

I want to perform electropolymerization on 3 system carbon electrode. How to cover the reference and counter electrode? I want to make polymer only on working electrode.

23 May 2024 1,681 3 View

Which books should i read to learn the basics of Magnetic Nanoparticles?

I want to learn the Magnetism of nanostructured materials in very deep. Please suggest me some books , if anyone of you have suggestions.

18 May 2024 7,415 1 View

Electro-optical Characteristics Measurement System- LCMS. Is the VT curve means graph between Driving voltage and relative transmittance?

After measuring VT curve using Electro-optical Characteristics Measurement System like LCMS, how to plot graph. What is PD voltage and relative transmitance. Is the VT curve means graph between...

15 May 2024 2,393 0 View

How to fix the molarity of 2 analytes to be interacted in Gauss View using DFT ?

I am trying to optimise the molar ratio of 2 analyte (For example A and B) during a reaction. For this purpose I want to optimise there molar ratio. For example A:B = 1:2, 1:3, 1:4, etc. Which...

28 April 2024 5,775 2 View

How to perform TEM imaging of chitosan nanoparticles using cooper coated TEM grids?

Is it possible to get a proper TEM images of chitosan nanoparticles as a film of nanoparticles are formed on the cooper grid?

16 April 2024 368 3 View

Can anyone help me in calculating surface coverage area of the 3 system electrode using electrochemical?

How do we calculate the surface coverage area of the electrode using electrochemical study?

17 March 2024 9,761 1 View

Is there any advice on the synthesis of carbon quantum dots?

Greetings to all, As part of my research, I am synthesising carbon quantum dots. Using ethanol as the solvent, I performed a solvothermal method. As I have tried different temperatures and times...

06 February 2024 1,459 3 View

Is there any alternative for freeze drying method for drying of chitosan polymer matrix?

Is there any alternative for a freeze-drying method for drying of the chitosan polymer matrix?

11 December 2023 1,902 4 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

What is the reason for the non-monotonic variation of roughness for sputtered Cu films annealed at 100, 200 and 300 degree Celsius?

I have prepared Copper thin films by magnetron sputtering and the samples have been annealed at 100, 200 and 300 degree celsius. The variation of the rms roughness and all other AFM height related...

11 June 2024 9,774 1 View

What are the laboratory precautions taken by the user when handling cancer cells or cancer cell derived extracellular vesicles?

Please give some insight on this

06 June 2024 2,016 2 View

Fix CO2 in charcoal and refill coal-mines?

1 ton charcoal fixes 3,7 t CO2. The global charcoal production is 54 mio tons, in which 200 mio t CO2 are fixed. 200 times the currently produced charcoal will fix the entire CO2 emitted globally...

29 May 2024 3,783 2 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View

Surface roughness in the planar optical waveguide induced propagation losses, any min or max limit for surface roughess to reduce propagation losses?

I am working on SiO2/Si3N4/SiO2 planar waveguide structure and characterized the Si3N4 (core layer, deposited at different sputtering powers)through AFM for RMS surface roughness. (in range of...

02 May 2024 1,343 0 View

How can I measure precisely the roughness of the sandpaper?

Other two questions are: 1) Are the conversion tables (grit-microns) found om internet reliable? 2) Where does the equation to convert grit in microns come from?

02 May 2024 3,329 1 View

Why do people still refer to female ejaculation?

In the younger boy there is no ejaculate because the prostate and seminal vesicles are not yet functionally developed, and in the female those glands are rudimentary and never develop. (Alfred Kinsey)

29 April 2024 6,483 0 View