How to capture UV- fluorescence from a substance using a camera?

I have a liquid that glows when UV light is shined. However, when I use my phone camera I can't see the glow, I just see a violet hue. What is the best way of capturing the fluorescence-glow from...

02 October 2020 8,664 3 View

What digital technologies are more helpful to sustain Agriculture?

With the global Corona Pandemic situation, it looks to be inevitable to move towards digital technologies to get adapted in various farming sectors. With the advent of IoT, Robotics, Data Science,...

10 May 2020 8,570 5 View

Are there reliable antibodies against TRP channels in cardiac cells ?

From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or...

07 May 2020 6,865 1 View

What are the applications of chemical solvent with composition Hexane 50% + Isopropyl alcohol 50%?

What are the applications of chemical solvent with composition Hexane 50% + Isopropyl alcohol 50%. Thank you very much.

04 May 2020 4,022 2 View

How to import the irradiation flux profile from the ray tracing software TracePro to ANSYS Fluent as a boundary condition for thermal analysis?

I am using TracePro software for optical simulation of my solar concentrator system. The software gives the results as irradiation flux map and also the incident ray table. Now for thermal...

28 March 2020 5,216 4 View

Wired ghost image- In a TEM image. What might be the cause?

I am wondering what that ghost thick outline, which is around the carbon wire image which I have attached. I did prepare my sample in a different method (sonication-dispersion in Acetone- and...

11 January 2020 3,054 10 View

How to interpret the XRD results to find the Chemical composition of the sample under study?

I have used EDX-SEM to identify the elements present in my powdered sample later I have used XRD got the results, now how to interpret the results to find the chemical compounds present in my...

31 October 2019 509 3 View

Is it possible to simulate the electrical output of a silicon PV cell using ANSYS ?

I am working on concentrated photovoltaic thermal system in which i need to simulate both its thermal and electrical output. Using ANSYS I am able to simulate the thermal output but how to do a...

31 October 2019 7,673 2 View

No/less features when electrodes tested in two electrode cell rather than 3 electrode cell?

Determination of the Specific Capacitance of Conducting Poly... Answers to my questions: Carbon-nanofiber electrodes directly grown on a nickel foam ......

22 October 2019 1,875 1 View

Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

05 March 2019 365 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View