Can we use luciferase assay cell lysate for western blot? if anybody has used, please share protocol?

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Hamadi Madhi

Dear Suruchi,

It is possible to use cell lysate for blotting after luciferase assay.However, your protein lysate might be digested by proteases as a result of manipulation and lysis at room temperature. For this purpose, it might be risky to do so.

Hope it helps.

Best,

Hamadi

Louis Tong

Yes, for the reasons geiven by Hamadi, if the lumescience is detected, it may not be of the expected molecular weight.