Can we use any whatmann filter papar or any other thick cellulose filter paper in western blotting?
Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out
07 August 2024 6,065 0 View
Answer should be based on institutional level
29 July 2024 1,504 4 View
I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...
19 July 2024 4,120 5 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?
04 June 2024 2,602 5 View
If we design proteins like tetrabody or triabody with unique binding sites in silico without further wet lab validation can we patent that structure and procedure?
01 June 2024 2,493 1 View
So, I am working on a small protein (X) of Haloferax volcanii. It shows these results: 1. Mutant strain grows poorlycompared to wild type strain under low iron condition. 2. Growth of Mutant...
14 May 2024 9,037 4 View
Hi I am Muhammad and working with an Sf9 cell line expressing neuropeptide receptors and using the cAMP-Glo™ Assay kit to measure the luminescent signal of these receptors. One of my cell lines is...
29 April 2024 8,564 0 View
My simulation suddenly stopped due to some reason. Now i cannot restart the simulation from previous checkpoint because it was not written properly. my _prev.cpt file is also corrupted....
18 April 2024 2,461 0 View
Please suggest bioinformatics journals which do not need wet lab experiments.
10 March 2024 2,919 6 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View
I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...
12 July 2024 8,116 2 View
NA
11 July 2024 8,967 1 View
Hello everyone! I would appreciate some advice regarding the filtering of structural variants. The variant caller used for discovery-“DELLY or DELLY2”using whole-genome sequencing and Whole...
09 July 2024 495 1 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View
I use X-ray diffraction (XRD) techniques to quantify the degree of crystallinity in my samples, referring to the following formula (CrI=Acrystal-Aamph/Atotal ). using deconvolution area methods (...
06 July 2024 6,233 9 View
Hello, I am looking for (estimated) degradation rates of cellulose in a classic anaerobic digestion setup (meso or thermophilic, WWTP sludge, 30+ days of operation) to have an idea of the % of...
01 July 2024 5,867 5 View
After finish the synthesis of nanoparticles.and nanoplates of silver how to take out those as solid/powder form from the solution. Please give a solution of u already done that and succeeded by that
27 June 2024 691 1 View