Can I alkylate (e.g. iodoacetamide) solvent exposed cysteines of a purified membrane protein??

I have purified a transporter membrane protein and want to perform a CPM assay to assess its melting temperature as apo- and with ligands. The melt scan results in steadily dropping curve while my...

26 September 2020 1,455 4 View

How do I compare various cox proportional hazard regression models in SPSS?

I have generated various models with an identical core of fixed covariates with each model differing only in the study covariate (a categorical variable that I change in each model by altering...

14 September 2019 2,397 2 View

Why in 211 MAX phase publications mainly Ti2AlC, Ti2AlN and Cr2AlC are considered?

There is a plethora of possible MAX phases, but from what i have seen, papers tend to focus mainly on those three. What about all the other ones? Are they experimentally not feasible?

12 March 2019 4,300 6 View

What does it mean if I find more than one STR in a sequence?

I used TandemRepeatFinder to find STRs in sequences I retrieved from Genbank and in some sequences the program has found more than one STR. What does this mean? In a sequence for example, some of...

30 March 2018 3,794 1 View

What is your opinion about Fritz Haber?

02 April 2017 6,948 1 View

How to aplpy periodic boundary conditions on a cubic rve of a cellular solid using ANSYS/APDL(any code,advice,example would be very useful)?

Any type of help (guidelines,advice codes,examples) would be much appreciated since i'm having a tough time applying PBC in Ansys(APDL).

04 July 2016 6,441 2 View

Can we use Busch theorem to find the beam radius and the Larmor radius at any axial position?

The question is fairly simple In the book "Gyrotrons: High-Power Microwave and Millimeter Wave Technology" from M. V. Kartikeyan, Edith Borie, Manfred Thumm, in page 107 a generalization of Busch...

21 May 2015 1,281 2 View

Is there a resource for the current Tree of Life, based on published research?

I need to access global phylogenies, going down to the species level if possible. Is there a resource that provides different resolutions of the Tree of Life, based on our current knowledge about it?

01 February 2014 6,647 7 View

Any suggestions for synchronizing TM4 Sertoli cells?

I've recently thawed a TM4 Sertoli cell line, which I'd like to stimulate with a specific substance, but before I do that I want to synchronize it by using a specific Hunger medium. I've read...

28 January 2014 1,877 3 View

If we were to consider a concept to be the irreducible quantum of consciousness, how can this be applied and used in Cognitive Neuroscience?

A concept is not a mere group of coactivating synapses or a pattern of neuronal activity. It is the recognizable awareness of this underlying event. I came to the realization that a concept may...

30 August 2013 8,109 34 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View