I ran an amplification reaction (regular PCR) and the reaction failed (the polymerase might have been left outside overnight). Anyhow no bands showed on the gel (not even for my positive control), and I have very little DNA left, so I am wondering if I can salvage the DNA used in that reaction by using a PCR clean up kit or will it have degraded by the end of the run if there was no amplification?
attached are the details of my run
Hi Maryam,
I would give it a try. Use a PCR cleaning kit to clean up and re-do PCR. You might get what you want since PCR is a more sensitive application.
To add to what DT Pathak already told you, I would add a fresh set of primers and Polymerase and try the PCR again with reduced Tm. Addition of small amount of primers and Pol wouldn't change the reaction volume much.
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India