Any recommendations for Epoxide Ring Opening?

Hi, I am looking for some books which have detail information on epoxide ring opening reactions in cyclic molecules like norbornene, cyclohexane, dicycopentadiene, etc. Please suggest me some...

04 May 2019 1,260 3 View

What factor governs the manufacturing of common inorganic acids in water?

I want to know that why we have fixed concentrations of HCl (37%), HBr (48%), H2SO4 (98%), etc. Why we cannot have all acids of same strengths or same concentrations?

02 March 2018 6,191 11 View

What is difference between Melt State, Liquid State and Solution State of a compound?

If I dissolve my compound in a solvent then how does its properties differ from its liquid state? Moreover, by liquid state I mean its melt state. So I am confused between Melt State, Liquid State...

31 December 2017 7,167 3 View

How can an Organic Silyl Hydride be separated from its corresponding Silanol from a mixture of both?

I have a mixture an organic silyl hydride and its corresponding silanol. I need to separate both of them. Which technique could be best possible to do so. I don't know the boiling points of either...

09 October 2017 3,743 2 View

What data is needed for an inseparable mixture of isomers of a novel new compound?

I have synthesized an organic compound which have no structural similarity with any of the existing once. But I am sure that the compound is a mixture of its isomers which is inseparable (I...

08 September 2017 3,162 5 View

How can we convert a thick semi solid material into a solid powder permanently?

06 July 2017 5,119 6 View

How can I remove 1,3-PropaneDiamine from reaction mixture?

I am performing Alkyne Zipper Reaction which requires 1,3-Propanediamine as a solvent. After completion of reaction, I need to extract my compound which is highly polar. But, since...

04 May 2017 7,585 11 View

How to produce solvated electrons in the reaction?

I want to perform a reaction, in which sodium metal should produce solvated electrons. But my limitations are that I cannot use Liquid Ammonia, Methylamine or Ethylamine etc. as solvent....

31 December 2016 6,669 6 View

What will be the best data to be produce for publication if the catalyst used in organic synthesis is a metal nanoparticle?

I need to give the data for Metal Nanoparticles which I have used as a catalyst for organic synthesis. So, as I know, DLS study can give size information. But will SEM or TEM images help in this...

10 November 2016 7,402 0 View

How can we remove DMF from a reaction mixture?

My reaction mixture has DMF and water in ratio 1:10 (DMF:Water). After the completion of reaction, I need to extract my compound which is highly polar. When, I use Dichloromethane or Ethyl...

01 January 1970 4,260 20 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View