Can we replace ISPF by commercial urea kit when doing arginase assay?

10 May 2016 0 3K Report

I am planning to do an arginase assay, but we can't have ISPF in our lab, can I replace it with a commercial urea kit? And what is the role of acid mixture? My understanding is that it stops the reaction of arginine metabolism, which would mean that even if I use a urea commercial kit I should maintain this step of the assay. Thanks in advance

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