Can we remove the primer dimer noise in the RT-qPCR by Tm calling analysis?

Justyna Augustyniak @Justyna-Augustyniak

20 July 2020 0 8K Report

Interesting me the possibility of remove the noise came from primer dimer from RT-qPCR data.

Can you explain to me is it possible and how it works?

Does Tm calling give me the possibility to get a more corrected CT value?

How to use the Tm calling option in the analysis of gene expression?

Thanks for your help!

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