Can we perform CRISPR-screening by using Cas9 (WT) with a sgRNA subpooled library designed to be used with dCas9 systems? Does it work?

Is it possible to use Cas9 (WT) to perform CRISPR-screening with a sgRNA subpooled library (Addgene catalog number: 83971) designed to be used with dCas9 systems? Beyond mutations at nuclease...

17 October 2022 2,425 1 View