I am working on lung inflammatory disorder which is marked by oxidative stress. In order to measure mitochondrial ROS in bronchoalveolar lavage fluid (BALF) sample procured from mice, I am planning to utilize mitoSOX probe. I have a query if we can directly use mitoSOX on BALF cells and measure the fluorescent intensity through flow cytometry? OR
Do we need to use antibodies (along with mitoSOX) against specific markers of inflammatory cells involved in lung inflammatory disease?
The following may be helpful to start with:
Article New Frontiers for Molecular Pathology
Article Melatonin induced suppression of ER stress and mitochondrial...
Article Critical role of mitochondrial oxidative stress in acid aspi...
BALF from infectious mice has both Macs and Neutrophils. So, If you want to compare ROS in a particular cell type, it is recommended to add a marker antibody in the experiment.
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